Nowadays, plant micropropagation is considered the main propagation method for numerous plant species, especially recalcitrant woody species, including the fig tree. However, micropropagation is still associated with some limitations, such as endophyte contaminations, phenolic compound secretion, and the lack of a reliable micropropagation protocol for many species. Thus, the main purpose of this study was to establish a reliable and efficient micropropagation protocol for F. carica cv. Sabz, as the most precious fig cultivar in Iran. For the elimination of endophytic bacteria contaminations, an initial experiment including different concentrations of ceftriaxone antibiotic (0, 50, 100, 150, 200 and 250 mg/l) was carried out. In the establishment stage, the effect of two basal media (MS and WPM) and PGRs combinations, including the interactions of BA (0, 0.5, 1, and 1.5 mg/l) × NAA (0 and 0.1 mg/l) were investigated. In the shoot proliferation stage, the MS medium and the effect of different concentrations of BA (0, 0.5, 1, and 2 mg/l) were evaluated. In the rooting stage, the effect of different concentrations of IBA (0, 0.5, 1, and 1.5 mg/l) and half-MS medium were evaluated. After four weeks (the end of the rooting stage), parameters of rooting percentage, root numbers, and root length were calculated. The results demonstrated that the endophytic bacteria were successfully eliminated by using 250 mg/l of ceftriaxone in the culture medium. MS medium supplemented with 0.5 mg/l BA with or without 0.1 mg/l NAA was found as the best establishment medium (100 %). Besides, MS medium enriched with 2 mg/l BA was identified as the best proliferation medium with a mean of 3.2 shoots/explant which displayed a significantly different from other proliferation treatments. ½ MS medium containing 1.5 mg/l IBA resulted in the highest rooting percentages (88.26%), root length (6.15 cm), and root numbers (8.46). |
