The reduction in the internal energy after harvesting of rose cut flowers accelerates the senescence process and prevents the full opening of the bud. In this experiment's effects of energy, suppliers included sucrose (1%, 2% and 3%), mannitol (100, 200 and 300 mM) and ATP (0.1, 0.3, 0.5 and 0.7 mM) on the vase life of Rosa hybrida cv. Samurai investigated, The results of continuous treatment with sources of energy supply showed that ATP treatment of 0.7 mM had the highest effect on the vase life of cut flowers (14.2 days), which showed 5.8 days increase in vase life compared with control treatment (8.2 days). ATP at 0.3, 0.5 mM concentrations increased the vase life of cut flowers to 11 and 12 days, respectively. Also, the vase life in sucrose 3% was 11.4 days. In the above treatments, solution uptake, relative fresh weight, and relative flower diameter were higher than other treatments. Besides, the activity of peroxidase enzyme and ion leakage of petal during the postharvest period was lower in treated cut flowers with concentrations of 0.7 and 0.5 mM ATP. Results showed that the use of different concentrations of mannitol did not increase the vase life of rose and had a negative effect on the quality of flowers so that the vase life of cut flowers was 1.8 days lower than the control treatment. Our results showed that the supply of energy for cut flowers by ATP treatments can be an effective way to maintain the longevity of rose cut flowers. Therefore, it is recommended to use of ATP (0.7 and 0.5 mM) in vase solution to maintain the postharvest quality of Rose cut flowers.