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Investigation of Nickel and Urea Interaction on morphological and biochemical characteristics of Roses (Rosa hybrida cv. Tonight) in Hydroponic cultivation
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Saber Shokri   , Parviz Noruzi , Rasoul Rahnemaie , Javad Rezapourfard |
| Urmia university |
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Abstract: (384 Views) |
Urea is one of the sources of nitrogen fertilizer, which has many applications in agriculture due to its low cost, easy application and high nitrogen content (46%). The main role of nickel is to participate in urea metabolism in plants with urea nitrogen source. This research was conducted with the aim of investigating the effect of nickel and urea nutrition in the hydroponic cultivation conditions on the growth characteristics and also reducing the negative effects of ammonium application in the winter season (decreasing the pH of the root environment and disrupting the absorption of elements) of cut roses of (Rosa hybrida cv. Tonight). A factorial experiment was performed based on completely randomized design with three levels of urea (0, 500 and 1000 µmol) and three levels of nickel (0, 2.5 and 5 µmol) from the source of nickel nitrate by directly adding it to the fertilizer solution. 3 months after applying the treatments, sampling of the developed leaves was done to analyze the morphological and biochemical traits. The results of this experiment showed that the interaction of nickel and urea was significant on the traits of steem height, flower length and flower diameter, fresh weight, steem diameter, chlorophyll and carotenoid content, total protein, anthocyanin and phenol. The application of 1000 (µmol L-1) of urea and 2.5 (µmol L-1) of nickel led to a 40% increase in yield compared to the control treatment. The highest stem height (86.96 cm) was obtained in the treatment of 1000 (µmol L-1) of urea and 2.5 (µmol L-1) of nickel. High concentrations of nickel led to leaf toxicity, reduction of anthocyanin, chlorophyll, and increase of total phenol, and as a result, reduced the green color of leaves. The highest amount of superoxide desmutase enzyme (0.577 U/mg Protein) was obtained in the treatment of 500 micromoles of urea and 2.5 micromoles of nickel, and the lowest amount of the enzyme (0.337 U/mg Protein) was gained in the treatment of 500 micromoles of urea without the presence of nickel. Application of urea alone had no significant effect on the above traits. By replacing ammonium with urea in the nutrient solution, it prevented the decrease in the pH of the environment.
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| Keywords: Anthocyanin, Phenol, Superoxide Dismutase, Biochemical |
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Full-Text [PDF 518 kb]
(177 Downloads)
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Type of Study: Applicable |
Subject:
Ornamental plants
Received: 2023/08/26 | Accepted: 2023/11/11 | Published: 2025/01/21
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