Physalis peruviana (also known as cape gooseberry, golden berry, or uchuva) is a member of the Solanaceae family, which is known for its unique organoleptic properties and medicinal metabolites of its fruits. Nowadays, it has been found that inoculating plants with plant growth-promoting rhizobacteria (PGPRs) can be a practical approach to increasing the quantity and quality of crops. The present project was conducted to assay the morphological and biochemical responses of P. peruviana to inoculation with two different PGPRs (Bacillus subtilis and Pseudomonas fluorescens). Our results showed that the inoculation with P. fluorescens led to an earlier initial harvest of P. peruviana. The highest whole plant fresh weight, yield, and fruit weight were measured in B. subtilis inoculated plants (2962 g per plant, 820. 62 g per plant, and 4.1 g, respectively). Both bacterial treatments increased the chlorophyll a and b content (27% and 20.8%, respectively, compared to control plants), and the highest total carotenoid content of P. peruviana leaves was measured in P. fluorescens -inoculated plants. Also, the inoculation with P. fluorescens led to the highest content of total phenolic compounds, total flavonoids, and total anthocyanins in P. peruviana fruits (28.95 mg GA/ 100 g FW, 1.55 mg quercetin/ 100 g FW and 1.28 µg cy-3-glu E/ 100 g FW). However, the differences between the two PGPR treatments weren’t significant in terms of total anthocyanins. Both B. subtilis and P. fluorescens inoculation increased the Phenylalanine ammonia-lyase enzyme activity by approximately 15% compared to control plants. Also, the highest beta-carotene and lycopene content in fruits was measured in P. fluorescens treatment (12.02 and 4.8 µg/g FW, respectively). However, the differences between the two PGPR treatments weren’t significant in terms of lycopene content. Our observation indicated that inoculation with both B. subtilis and P. fluorescens elevated the total antioxidant capacity of P. peruviana fruits. The IC50 index of total antioxidant capacity in DPPH free radical scavenging in P. fluorescens treated fruits (as the highest measured antioxidant capacity) was 19.40 (μg/ml).