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Impact of Auxin and Cytokinin on in vitro Culture of Asiatic Gotu Kola [(Centella asiatica (L.)] Urban
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Fereshteh Heidargholinezhad   , Yousef Hamidoghli , Valiollah Ghasemiomran , Pouria Biparva |
| Guilan University |
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Abstract: (1254 Views) |
Gotu Kola (Centella asiatica L.) has been listed in threatened species in Iran. It has been used for treatment of a wide number of health disorders. The herb was used for wound healing, anticarcinogenic, mental illness and revitalizing the nerves and brain cells. In vitro culture and micropropagation are basic tools for conserving this medicinal plant. The purpose of this study is to achieve a suitable protocol for propagation of this plant in in vitro. In the first experiment, interaction of different concentrations of BAP (0, 0.5, 1, 1.5, 2.5, 3.5 mgl-1) and NAA (0, 0.25, 0.5, 1, 2 mgl-1) were examined on leaf explants. The results of the callogenesis experiment showed that MS medium with 1.5 mgl-1 BAP and 0.5 mgl-1 NAA, produced the best callus. In the next step, the interaction of BAP at 0, 1, 2, 3 mgl-1 and IBA at 0, 0.5, 1 mgl-1 was investigated in proliferation of node explants. Rooting of amplified explants was investigated by using two types of auxin IBA and NAA. The results of the micropropagation experiment indicated that MS medium of 2 mgl-1 BAP and 1 mgl-1 IBA was determined the most suitable medium for micropropagation. In the comparison of the effect of auxin treatments in MS medium on rooting, MS medium supplemented with IBA 1 and 2 mgl-1 were chosen as the best rooting treatment. |
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| Keywords: Callus induction, Gotucola, Medicinal plant, Micropropagation, Tissue culture |
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Full-Text [PDF 679 kb]
(127 Downloads)
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Type of Study: Research |
Subject:
Medicinal plants
Received: 2022/09/3 | Accepted: 2022/12/26 | Published: 2024/05/19
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