The use of organic and biological fertilizers is important for achieving sustainable agriculture, reducing the application of chemical fertilizers and the production of healthy crops. A greenhouse experiment was carried out to study the effects of vermicompost, Nitragin, and nitrogen on some growth parameters of strawberry (Fragaria ananassa Duch. cv. Aromas). Treatments were arranged in a factorial experiment based on a completely randomized design with three replications. Treatments consisted of nitrogen fertilizer (0, 50, 75, and 100 kg ha^-1 nitrogen equal to 0, 75, 112.5 and 150 mg N pot^-1), vermicompost (0, 0.5%, and 1% w/w), and Nitragin (inoculation and non-inoculation). Nitragin application increased total fruit yield by 8.5 percent. The highest total fruit yield (71.8 g pot^-1) obtained by application of 75 kg ha^-1 along with 0.5% vermicompost and inoculation with Nitragin and the lowest one (13.3 g pot^-1) obtained in control treatment. The highest dry matter (6.06 g) obtained with co-application of 100 kg ha^-1nitrogen, 1% vermicompost, and inoculation with nitragin. The effects of the application of 75 kg ha^-1nitrogen fertilizer with vermicompost and nitragin were similar to the single application of 100 kg ha^-1nitrogen. Biofertilizers application may increase plant yield and reduce nitrogen fertilizer usage (up to 25%).