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Comparison of the Efficacy of Two Chloroplast DNA Marker (psbA-trnH and trnL-F) in Determining the Phylogenetic Relationships among Honeysuckle Species in Iran
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Najmeh Fattahi Dehkordi   , Masood Ghaemi Ghehsareh , Behrooz Shiran , Majid Siampoor |
| Shahrekord University |
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Abstract: (857 Views) |
The genus Lonicera belongs to the Caprifoliaceae family, and in Persian, it is called Palakhor, Shung, or Aminoddoleh. Understanding interspecific relationships based on traditional methods such as morphological features and chemical compositions has conflicting results. In this study, to evaluate the efficiency of DNA barcode in determining the phylogenetic relationships of 12 species of Lonicera under cultivation in different regions of Iran, two chloroplast DNA barcodes (psbA-trnH and trnL-F) were used. Genomic DNA extracted using available kits, and PCR reaction performed to amplify two regions, and finally, purified samples were sequenced. The results showed that the length of the psbA-trnH and trnL-F region are about 420-435 and 940-950 nucleotides, respectively. Parsimony analysis of psbA-trnH and trnL-F showed 382 and 886 conserved sites, 55 and 71 variable sites, and 15 and 16 Parsimony informative sites, respectively. Phylogenetic trees created by the psbA-trnH and the trnL-F regions showed that most species (except L. korolkovii) and the two species L. korolkovii and L. maackii were well distinguished from each other, respectively and they were placed in different clusters. The genetic distance (0.00 to 0.209) between species in psbA-trnH region is more than genetic distance (0.00 to 0.027) in trnL-F region. Therefore, the psbA-trnH region owing to its diversity and reproducibility is a suitable barcode for studying genetic diversity between Lonicera species. |
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| Keywords: Barcode regions, Phylogeny, Molecular marker, Honeysuckle |
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Full-Text [PDF 841 kb]
(127 Downloads)
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Type of Study: Research |
Subject:
Biotechnology and Tissue culture
Received: 2021/01/29 | Accepted: 2021/06/13 | Published: 2022/05/7
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