The Effect of Bud Position and Growth Regulators on the Micropropagation of Rosa damascena Mill.

Hassanpouraghdam, Mohammad Bagher; Ebrahimzadeh, Asghar; Aazami, Mohammad ali; Malekian, Sattar; Salehzadeh, Mehrdad; Vojodi Mehrabani, lamia

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Volume 23, Issue 3 (Fall 2022)

IJHST 2022, 23(3): 485-496

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The Effect of Bud Position and Growth Regulators on the Micropropagation of Rosa damascena Mill.

Mohammad Bagher Hassanpouraghdam

, Asghar Ebrahimzadeh

, Mohammad ali Aazami

, Sattar Malekian

, Mehrdad Salehzadeh

, Lamia Vojodi Mehrabani

University of Maragheh, and Islamic Parliament Research Center, Tehran, Iran

Abstract: (508 Views)

Several experiments were conducted to study the effects of bud position and plant growth regulators on the micropropagation of Rosa damascena. In the first experiment, the effects of bud localities (the proximal, middle, distal and shoot tip), BAP (2.5 and 5 mgL^-1), TDZ (0.05 and 0.1mgL^-1) and NAA (0.1 mgL^-1) were studied on the regeneration of plant. The highest shoot length in the distal, middle and proximal bud locations was attained by 2.5 and 5 mgL^-1 BAP + 0.1 mgL^-1 NAA. For the proximal and shoot tips, the top data was related to 0.05 mgL^-1of TDZ + 0.1 mgL^-1 NAA. In the second experiment, Kin and BAP (0, 1.5, 3 and 4.5 mgL^-1) treatment combinations were tested on the regeneration potential without considering bud location. The highest shoot length belonged to 4.5 mgL^-1of BA and BAP. For the rooting stage; 0.1, 0.2 and 2 mgL^-1of NAA and IBA were examined and the highest rooting percentage and root length was recorded for 0.1 mgL^-1IBA. In conclusion, the in vitro regeneration response was controversy for the bud location. However, the growth regulators had promising effects on the regeneration of Rosa damascena.

Keywords: BAP (6- benzyl amino purine), NAA, TDZ, Rooting

Full-Text [PDF 585 kb] (126 Downloads)

Type of Study: Research | Subject: Biotechnology and Tissue culture
Received: 2020/10/22 | Accepted: 2021/08/26 | Published: 2022/12/25

References

1. Adhikari, S.R. and B. Pant. 2013. Induction and proliferation of in vitro mass of callus of Withania somnifera (L.) Ann. Res. Plant Sci. 1 (3): 58 - 61.

2. Arnold, N.P., M.R. Binns, N.N. Barthakur and D.C. Cloutier. 1992. A study of the effect of growth regulators and time of plantlet harvest on in vitro multiplication rate of hardy and hybrid tea roses. J. Hort. Sci. 67: 727-35. [DOI:10.1080/00221589.1992.11516303]

3. Badzhelova, V. and V. Bozhanova. 2018. Efficient protocol for in vitro clonal propagation of Rosa amascena Mill. and its comparison with conventional propagation method. Bulg. J. Crop Sci. 55(6): 37-44.

4. Baiest, M.M.H., S.N. Azimi, N. Mortazavi and H. Mohammadi. 2013. Study of the effects of different concentration of IBA and 2.4.D on the rootting Rosa damascene cuttings. National Symposium of Passive Defence in Agricultural Section, Qeshm. (In Persian).

5. Carelli, B.P. and S. Echeverrigary. 2002. An improved system for the in vitro propagation of rose cultivars. Sci. Hort. 92: 64 - 74 [DOI:10.1016/S0304-4238(01)00280-1]

6. Debener, T. and L.H.S. Oyant. 2009. Genetic engineering and tissue Culture of Roses. Plant Physiol. 6: 393 - 409. [DOI:10.1007/978-0-387-77491-6_19]

7. Doina, C., A. Fira, O. Borsai, M. Hart, C. Sisea, R. Pop and D. Pamfil. 2017.Micropropagation of Rosa damascena Mill: the effects of gelling agents on the multiplication stages and acclimatization. Agri. Sci. Practice. 3(4): 56-63.

8. Ginova, A., I. Tsvetkov and V. Kondakova. 2012. Rosa damascena Mill. An overview for evaluation of propagation methods. Bulg. J. Agr. Sci. 18: 545 - 556.

9. Huettman, C.A. and J.E. Preece. 2000. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plan Cell Tissue Organ. Cult. 33: 105 - 119. [DOI:10.1007/BF01983223]

10. Islam, M. M., M. Ahmed and D. Mahaldar. 2005. In Vitro callus induction and plant regeneration in seed explants of Rice (Oryza Sativa L.). Res. J. Agr. Biol. 72 - 75.

11. Khosh-Khui, M. 2014. Biotechnology of Scented Roses: A Review. Int. J. Hort. Sci. Tech. 1(1): 1-20.

12. Kornova, K.M. and J. Michailova. 2000. Study of in vitro rooting of Kazanlak oil-Bearing (Rosa damascena Mill.). J. Essent. Oil Res. 6: 485 - 492. [DOI:10.1080/10412905.1994.9698431]

13. Kumar, A., A. Soda, U. Palni, A. Gupta and L. Mankok Palni. 2001. Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron. J. Hort. Sci. Biotech. 76(1): 30-34. [DOI:10.1080/14620316.2001.11511322]

14. Omidbaigi, R. 1995. Approaches to Production and Processing of Medicinal Plants. Fekr-e-Roz Publication, P.P. 283. (In Persian).

15. Omidi, M. And A. Yadollahi. 2016. Factors affecting in vitro propagation efficincy of three genotype of Rosa damascena. J. Bio. Nat. 6(2): 78-87.

16. Pati, P.K., M. Sharma, A. Sood and P.S. Ahuja. 2009. Micropropagation of Rosa damascena and R. bourboniana in liquid cultures. Bio. Plant. 48: 609 - 611. [DOI:10.1023/B:BIOP.0000047161.16944.b3]

17. Peivandi, M., L. Kazemi and A. Majd. 2015. The effects of different cytokinins on the proliferation of Lavandula vera. J. Plant Res. (Iran. J. Biol.). 28 (2): 257-263. (In Persian).

18. Piri, K.H., M. Hasandokht and R. Abrishami. 2006. Plant tissue culture guide. Plant. Cell. Tissue. Organ. Culture. 33: 105 - 119.

19. Ranjbar, A. and A. Nourollah. 2016. Improving the rooting of two Rosa damascene genotypes cutting. Flower Ornam Plants, 2(1): 59-68. (In Persian).

20. Rout, G.R., A. Mohapatra and S. Mohan Jain. 2006. Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects. Biotechnol. Adv. 24: 531-560. [DOI:10.1016/j.biotechadv.2006.05.001]

21. Sabri, N. 1994. Cytogenetic and electro pheretic study of the taxon, Vinca; tissue and cell culture, for the production of alkaloid chemotypes, MSc thesis. Tarbiat Modares University. Iran. (In Persian).

22. Tabesh, F., M. Jafarkhani Kermani, S.M. Khayam Nekuie and A. Mosavi. 2013. In vitro propagation of damask rose (Rosa damascena cv. Isphan). Ann. Biol. Sci. 4(8): 134 - 138. [DOI:10.3923/ijar.2013.172.177]

23. Wojtania, A., and B. Matysiak. 2018. In vitro propagation of Rosa Konstacin (R. Rugos × R. Beggerana) a plant with high nutritional and pro-health value. Folia. Hort. 30(2): 259-567. [DOI:10.2478/fhort-2018-0022]

24. Zarghari A. 1988. Medicinal Plants. Tehran University Publication. Volume 2. PP. 248. (In Persian).

25. Zoulfaghari-nasab, R., M. Khosrou-shahli, V. Grigourian and A.R. Motalebi Azar. 2004. Investigation of in vitro propagation of Apricot × Plum natural hybrids. Iranian J. Hort. Sci. Tech. 5(5): 81-92. (In Persian).

26. Adhikari, S.R. and B. Pant. 2013. Induction and proliferation of in vitro mass of callus of Withania somnifera (L.) Ann. Res. Plant Sci. 1 (3): 58 - 61.

27. Arnold, N.P., M.R. Binns, N.N. Barthakur and D.C. Cloutier. 1992. A study of the effect of growth regulators and time of plantlet harvest on in vitro multiplication rate of hardy and hybrid tea roses. J. Hort. Sci. 67: 727-35. [DOI:10.1080/00221589.1992.11516303]

28. Badzhelova, V. and V. Bozhanova. 2018. Efficient protocol for in vitro clonal propagation of Rosa amascena Mill. and its comparison with conventional propagation method. Bulg. J. Crop Sci. 55(6): 37-44.

29. Baiest, M.M.H., S.N. Azimi, N. Mortazavi and H. Mohammadi. 2013. Study of the effects of different concentration of IBA and 2.4.D on the rootting Rosa damascene cuttings. National Symposium of Passive Defence in Agricultural Section, Qeshm. (In Persian).

30. Carelli, B.P. and S. Echeverrigary. 2002. An improved system for the in vitro propagation of rose cultivars. Sci. Hort. 92: 64 - 74 [DOI:10.1016/S0304-4238(01)00280-1]

31. Debener, T. and L.H.S. Oyant. 2009. Genetic engineering and tissue Culture of Roses. Plant Physiol. 6: 393 - 409. [DOI:10.1007/978-0-387-77491-6_19]

32. Doina, C., A. Fira, O. Borsai, M. Hart, C. Sisea, R. Pop and D. Pamfil. 2017.Micropropagation of Rosa damascena Mill: the effects of gelling agents on the multiplication stages and acclimatization. Agri. Sci. Practice. 3(4): 56-63.

33. Ginova, A., I. Tsvetkov and V. Kondakova. 2012. Rosa damascena Mill. An overview for evaluation of propagation methods. Bulg. J. Agr. Sci. 18: 545 - 556.

34. Huettman, C.A. and J.E. Preece. 2000. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plan Cell Tissue Organ. Cult. 33: 105 - 119. [DOI:10.1007/BF01983223]

35. Islam, M. M., M. Ahmed and D. Mahaldar. 2005. In Vitro callus induction and plant regeneration in seed explants of Rice (Oryza Sativa L.). Res. J. Agr. Biol. 72 - 75.

36. Khosh-Khui, M. 2014. Biotechnology of Scented Roses: A Review. Int. J. Hort. Sci. Tech. 1(1): 1-20.

37. Kornova, K.M. and J. Michailova. 2000. Study of in vitro rooting of Kazanlak oil-Bearing (Rosa damascena Mill.). J. Essent. Oil Res. 6: 485 - 492. [DOI:10.1080/10412905.1994.9698431]

38. Kumar, A., A. Soda, U. Palni, A. Gupta and L. Mankok Palni. 2001. Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron. J. Hort. Sci. Biotech. 76(1): 30-34. [DOI:10.1080/14620316.2001.11511322]

39. Omidbaigi, R. 1995. Approaches to Production and Processing of Medicinal Plants. Fekr-e-Roz Publication, P.P. 283. (In Persian).

40. Omidi, M. And A. Yadollahi. 2016. Factors affecting in vitro propagation efficincy of three genotype of Rosa damascena. J. Bio. Nat. 6(2): 78-87.

41. Pati, P.K., M. Sharma, A. Sood and P.S. Ahuja. 2009. Micropropagation of Rosa damascena and R. bourboniana in liquid cultures. Bio. Plant. 48: 609 - 611. [DOI:10.1023/B:BIOP.0000047161.16944.b3]

42. Peivandi, M., L. Kazemi and A. Majd. 2015. The effects of different cytokinins on the proliferation of Lavandula vera. J. Plant Res. (Iran. J. Biol.). 28 (2): 257-263. (In Persian).

43. Piri, K.H., M. Hasandokht and R. Abrishami. 2006. Plant tissue culture guide. Plant. Cell. Tissue. Organ. Culture. 33: 105 - 119.

44. Ranjbar, A. and A. Nourollah. 2016. Improving the rooting of two Rosa damascene genotypes cutting. Flower Ornam Plants, 2(1): 59-68. (In Persian).

45. Rout, G.R., A. Mohapatra and S. Mohan Jain. 2006. Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects. Biotechnol. Adv. 24: 531-560. [DOI:10.1016/j.biotechadv.2006.05.001]

46. Sabri, N. 1994. Cytogenetic and electro pheretic study of the taxon, Vinca; tissue and cell culture, for the production of alkaloid chemotypes, MSc thesis. Tarbiat Modares University. Iran. (In Persian).

47. Tabesh, F., M. Jafarkhani Kermani, S.M. Khayam Nekuie and A. Mosavi. 2013. In vitro propagation of damask rose (Rosa damascena cv. Isphan). Ann. Biol. Sci. 4(8): 134 - 138. [DOI:10.3923/ijar.2013.172.177]

48. Wojtania, A., and B. Matysiak. 2018. In vitro propagation of Rosa Konstacin (R. Rugos × R. Beggerana) a plant with high nutritional and pro-health value. Folia. Hort. 30(2): 259-567. [DOI:10.2478/fhort-2018-0022]

49. Zarghari A. 1988. Medicinal Plants. Tehran University Publication. Volume 2. PP. 248. (In Persian).

50. Zoulfaghari-nasab, R., M. Khosrou-shahli, V. Grigourian and A.R. Motalebi Azar. 2004. Investigation of in vitro propagation of Apricot × Plum natural hybrids. Iranian J. Hort. Sci. Tech. 5(5): 81-92. (In Persian).

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Hassanpouraghdam M B, Ebrahimzadeh A, Aazami M A, Malekian S, Salehzadeh M, Vojodi Mehrabani L. The Effect of Bud Position and Growth Regulators on the Micropropagation of Rosa damascena Mill.. IJHST 2022; 23 (3) :485-496
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