An experiment carried out to study the effects of arbuscular mycorrhizal fungi on growth and physiological parameters ofthreecommercialapple clonalrootstocks during growing season in greenhouse. This investigation was conducted with two factors, rootstock (M₉, M₇ and, MM₁₀₆) and species of arbuscular mycorrhizal fungi (Glomus versiforme, Rhizophagus intraradices, Claroideoglomus etunicatum and, control) in a completely randomized design. Growth (including plant height and diameter and shoot fresh weight) and physiological (including total chlorophyll, photosynthesis, and transpiration) parameters as well as root colonization were measured in 11, 15 and 19 weeks after transplanting. Results showed that mycorrhizal treatments and rootstoks had significant effects on all measured parameters comparing to the control. It was also shown that the fungus Glomus versiforme performed better than other fungi having the highest records for almost all of the measured parameters. Among the rootstocks, MM₁₀₆ performed the highest shoot fresh weight (as well as plant height in the first and second measurements) but the final height of M₇ was more than MM₁₀₆. The highest and the lowest plant diameter were observed in MM₁₀₆ and M₇, respectively. The highest chlorophyll content, transpiration and photosynthesis rate was measured in M₉ but the highest root colonization was recorded in M₇. It seems that choosing proper rootstocks and treating them with suitable arbuscular mycorhizal fungi could reduce adverse conditions of calcareous soils for apple trees.