Winter freezing injury is one of the main problems for pomegranate production in Iran. Therefore evaluation of cultivars and different methods to overcome freezing injury is important. For this reason, it is necessary to find appropriate and reliable indices for evaluation of freezing damage in pomegranate trees. In present study cutting survival percentage, relative electrolyte leakage (EL), relative phenolic leakage and tissue coloring by tetrazolium chloride (TCC) indices were used for comparison of thin or thick of one or two year-old pomegranate shoots in freezing condition (-10, -15 and -20 ^oC). All evaluation indices except TCC showed the least freezing injury observed in one year-old thick shoots (LT₅₀= -15.4 ^oC). These shoots had the maximum amount of soluble sugars and proline content. However, there were no difference in relation t