Optimal planting pattern and proper nutritional management, especially the vital element of nitrogen, are among the most important and influential factors on the quantitative and qualitative traits of tomatoes. Two experiments were performed in two regions in Agricultural Research Stations of Bushehr Province in 2017-18 crop year in the form of split plots in a randomized complete block design with three replications. The main plot consisted of two planting patterns (single row and twin row) and the sub-plot consisted of four nitrogen levels (0, 90, 180 and 270 kg ha^-1 nitrogen). The results showed that the interaction effect of nitrogen × planting pattern in Bondarooz region had a significant effect on single plant yield, vitamin C, pH and partial factor productivity of nitrogen. In Saadabad region, the interaction of planting pattern and nitrogen on the number of locules per fruit and total soluble solids was significant. In Bondarooz, the highest and lowest single plant yields were obtained by application of 180 kg ha^-1 nitrogen in single row planting method and without nitrogen application in twin row planting method, respectively. In Saadabad, application of 270 kg ha^-1 nitrogen increased the yield of single plant by 116% compared to no application of fertilizer. The most important reason for the difference in yields between two regions was the amount of soil organic matter.