fa بررسی ترکیبات فیتوشیمیایی و پتانسیل آنتی‌اکسیدانی عصاره‌های اتانولی و اتیل‌استاتی سه اکوتیپ گیاه دارویی افسنطین Artemisia absinthium گیاهان دارویی Medicinal plants پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:14.2pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از جمله گیاهان با ارزش ایران، گونه‌های</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">جنس</span></span> <i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Artemisia </span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">متعلق</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تیره</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Asteraceae </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">می‌باشند</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">که</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پراکندگی نسبتاً</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">وسیعی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نقاط</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مختلفی از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ایران</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دارند.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پتانسیل</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آنتی</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکسیدانی به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">میزان</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">قابل</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">توجهی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ماهیت</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">حلال،</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">استخراج گیاه جمع‌آوری شده از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هر</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">منطقه، بستگی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پارامترهای</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">زیادی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ازجمله</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آب</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هوا،</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ارتفاع</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نور دارد. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه، گیاه دارویی افسنطین با نام علمی </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Artemisia absinthium</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از 3 ناحیه مختلف ایران جمع‌آوری و پتانسیل آنتی‌اکسیدانی این اکوتیپ‌ها ارزیابی شد. در این پژوهش خواص دارویی ازجمله فعالیت آنتی‌اکسیدانی (با دو روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">DPPH</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FRAP</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، مقدار فنول‌ و فلاونوئید کل در عصاره‌های قطبی (اتانول) و نیمه‌قطبی (اتیل‌استات)، درصد و تنوع ترکیبات اسانس مورد</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ارزیابی قرار گرفت. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکوتیپ گیلان دارای بیشترین فعالیت آنتی‌اکسیدانی بود و در مجموع اکوتیپ سمنان کمترین بازداری آنتی‌اکسیدانی را از خود نشان داد و حلال نیمه‌قطبی اتیل‌استات فعالیت احیاکنندگی بالاتری را نشان داد، که این مورد بیانگر این است که نوع حلال مورد استفاده در عصاره‌گیری بر فعالیت آنتی‌اکسیدانی بسیار مؤثر می‌باشد. در اکوتیپ‌های بررسی شده از دامنه 65/78 تا 58/92 درصد در روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">DPPH</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و از 76/11 تا 88/18 (در میلی‌مول‌آهن) در روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FRAP</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به‌دست آمد. اکوتیپ گیلان دارای بیشترین محتوای فنولی در عصاره قطبی و اکوتیپ گلستان بیشترین محتوای فلاونوئیدی را در عصاره نیمه‌قطبی نشان داد. اکوتیپ سمنان بیشترین ترکیبات مونوترپن‌اکسیژن‌دار و بیشترین ترکیب در اسانس را در بین دیگر اکوتیپ‌ها دارا بود. اکوتیپ گلستان دارای بیشترین درصد اسانس در بین دیگر اکوتیپ‌ها بود. تنوع محتویات فنولی، ترکیبات اسانس و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آنتی‌اکسیدانی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مشاهده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شده در</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سه اکوتیپ</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مورد</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مطالعه</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">می‌تواند</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به علت</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">عوامل</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مختلف</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکولوژیکی،</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژنتیکی،</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">جغرافیایی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">فاکتورهای</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تغذیه‌ای</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">باشد</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span></div> <br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">Among the valuable plants of Iran, the <i>Artemisia</i> species belongs to the Asteraceae family, which is relatively widely distributed in different parts of Iran. Considering that the antioxidant potential is significantly dependent on the nature of the solvent, the extraction method of the plant collected from each region also depends on many parameters such as weather, altitude, and light. In this study, wormwood (<i>Artemisia absinthium</i>) was collected from three different regions of Iran and the antioxidant potential of these ecotypes was evaluated. In this research, the medicinal properties, including the antioxidant content (by DPPH and FRAP assays), flavonoid content (TFC), and total phenol (TPC) were evaluated in polar (ethanol) and semi polar (ethyl acetate) extracts, and the percentage and variety of essential oil compounds were evaluated. The Gilan ecotype has the most antioxidants, The Semnan ecotype showed the least antioxidant inhibition, and the non-polar solvent ethyl acetate showed a higher regenerating activity, which indicates that the type of solvent used in extraction is very effective for antioxidant activity. The AC values of the ecotypes ranged from 78.65 to 92.58 % extract (by DPPH assay) and from 11.76 to 18.88 Mmol Fe (by FRAP assay) respectively. Gilan ecotype has the highest phenolic content in polar extract, and the Golestan ecotype showed the highest flavonoid content in non-polar extract. Semnan ecotype had the most oxygenated monoterpene compounds, and the most composition in essential oil among other ecotypes. Golestan ecotype had the highest percentage of essential oil among other ecotypes. The diversity of phenolic contents, essential oil, and antioxidant compounds observed in the three studied ecotypes can be due to various ecological, genetic, geographical, and nutritional factors.&nbsp;</span></span></span></span></span></span><br> &nbsp;</div> افسنطین, DPPH, FRAP, عصاره قطبی, عصاره نیمه قطبی Artemisia absinthium, DPPH, FRAP, Polar extract, Semi polar extract 345 358 http://journal-irshs.ir/browse.php?a_code=A-10-879-3&slc_lang=fa&sid=1 Sepideh Houshmand سپیده هوشمند sepidpam@yahoo.com 10031947532846009798 10031947532846009798 No دانشکده کشاورزی دانشگاه تبریز Saeideh Alizadeh- Salteh سعیده علیزاده سالطه s.alizadeh@tabrizu.ac.ir 10031947532846009799 10031947532846009799 Yes دانشکده کشاورزی دانشگاه تبریز Sahebali Bolandnazar صابحعلی بلند نظر bolandnazar@tabrizu.ac.ir 10031947532846009800 10031947532846009800 No دانشکده کشاورزی دانشگاه تبریز Elyas Aryakia الیاس آریاکیا aryakia@ibrc.ir 10031947532846009801 10031947532846009801 No بانک گیاهی، مرکز ملی ذخایر ژنتیکی و زیستی ایران