[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Indexing and Abstracting::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 21, Issue 3 (Fall 2020) ::
IJHST 2020, 21(3): 309-318 Back to browse issues page
Micropropagation of Gotu Kola (Centella asiatica L.) via Runner Tip Culture
Mohammadvali Habibi silabi , Yousef Hamidoghli , Amir Sahraroo
University of Guilan,
Abstract:   (1088 Views)
Gotu kola (Centella asiatica L.) is one of the most valuable medicinal plants. Due to the reduction of surface water and the limitation of dispersion, the natural populations of this plant species are in extinction risk. The purpose of this experiment was to introduce a tissue culture protocol for rapid micropropagation of the Gotu kola plant. For callus induction, explants were established on Murashige and Skoog (MS) medium supplemented with different concentration of 6-benzylamino purine (BA) at six levels (0, 1, 2, 3, 4, and 5 mgL-1) and α-naphthaleneacetic acid (NAA) at four levels (0, 0.25, 0.5, and 1 mgL-1). The best callogenesis was obtained with maximum callus induction (83.33%), fresh weight (4.26 g), and daily growth rate (0.14 g/day) by combined application of 4 mg L-1 BA and 1 mg L-1 NAA. Different concentrations of BA (0, 1, 2, 3, 4 and 5 mgL-1) and NAA (0.1 mg L-1) were used for shoot regeneration. Combination of BA (4.0 mgL-1) with NAA (1.0 mgL-1) showed the highest shoot proliferation (74.99%), maximum number of shoots (7.66), petiole (6.66) and the longest shoots (1.56 cm). The effects of, IBA (0, 0.5, 1, 1.5, 2, 2.5 mgL-1) were examined on adventitious root formation. MS medium supplemented with IBA (1 mgL-1) showed the best percentage of rooting (84.66%), maximum number of roots Per plantlet (65), and the longest roots (3.33 cm) along with the highest percentage of survival (74.99%).
Keywords: Auxin, Cytokinin, Callus induction, Tissue culture, Medicinal plant
Full-Text [PDF 375 kb]   (339 Downloads)    
Type of Study: Research | Subject: Medicinal plants
Received: 2019/08/1 | Accepted: 2020/06/25 | Published: 2020/10/4
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

habibi silabi M, hamidoghli Y, Sahraroo A. Micropropagation of Gotu Kola (Centella asiatica L.) via Runner Tip Culture. IJHST 2020; 21 (3) :309-318
URL: http://journal-irshs.ir/article-1-383-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 3 (Fall 2020) Back to browse issues page
مجله علوم و فنون باغبانی ایران Iranian Journal of Horticultural Science and Technology
Persian site map - English site map - Created in 0.05 seconds with 37 queries by YEKTAWEB 4645