[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Indexing and Abstracting::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 19, Issue 3 (Fall 2018) ::
IJHST 2018, 19(3): 341-352 Back to browse issues page
Effects of using Potato Black Dot Pathogen on Regeneration Resistant Stocks under in Vitro Conditions
Khosro Parvizi , Aziz Bagheri
Horticulture Crops Research Department, Hamedan Agricultural and Natural Resources Research and Education Center, AREEO, Hamedan, Iran.
Abstract:   (2234 Views)
In order to screen and induce the resistance of the potato stocks to the Colletotrichum coccodes, firstly, healthy seed tubers from two potato cultivars (Marfona and Agria) were placed in suitable conditions to produce sprout and single node. Then, segments of sprouts and single node of 5 to 7 mm were prepared. These segments were transferred to sterilized culture media containing MS and Nitsch & Nitsch mixed with three-dosage mixture of the fungus extract. Flasks of sprouts and nodal segment cuttings transferred into the chamber growth under appropriate temperature and light period until shoots were regenerated. This test arranged by factorial experiment based on randomized complete design with four replications. The factors were selected by cultivar in two level (Agria, Marfona), three concentration of fungus extract; low concentration (Six cc pure fungus extracts plus one volume of sterilized distilled water), Medium concentration (Six cc pure fungus extract plus one third volume of distilled sterilized water) and high concentration (Six cc pure fungus extract), distilled water as control treatment and culture medium in two levels (MS and Nitch & Nitch). After regeneration and production of new plantlets, their contamination and their health were evaluated. The result of the experiment showed that regeneration was not observed in sprout segments in both culture media and in both cultivars with all three concentrations of fungal extract, and only in the control treatment, the sprout segments were able to regenerate. However, regeneration was performed in the single nodes in both cultivars with a low concentration and average fungus extract.
 




 
Keywords: Fungus extract, Single node, Sprout, Tissue culture
Full-Text [PDF 236 kb]   (1023 Downloads)    
Type of Study: Research | Subject: Biotechnology and Tissue culture
Received: 2017/11/8 | Accepted: 2018/11/28 | Published: 2019/05/4
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Parvizi K, Bagheri A. Effects of using Potato Black Dot Pathogen on Regeneration Resistant Stocks under in Vitro Conditions. IJHST 2018; 19 (3) :341-352
URL: http://journal-irshs.ir/article-1-163-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 19, Issue 3 (Fall 2018) Back to browse issues page
مجله علوم و فنون باغبانی ایران Iranian Journal of Horticultural Science and Technology
Persian site map - English site map - Created in 0.05 seconds with 37 queries by YEKTAWEB 4645